Venture capital Zero One Hundred has announced the launch of its second fund, a €25 million early-stage vehicle focused on bridging Central and Eastern Europe (CEE) and the Middle East and North Africa (MENA).
Building on the track record of its first fund (which backed 24 startups and recorded two exits, with two more in progress) Fund II will allocate 70% of its capital to leading pre-seed and seed rounds in CEE, while 30% will be reserved for co-investments in MENA.
The fund is backed by a mix of institutional investors, exited founders, and seasoned entrepreneurs from across Europe and the Middle East.
Alongside the launch, Zero One Hundred has already made five new investments spanning FoodTech, PropTech, MedTech, HRTech, and AI:
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- Biteberry (Slovakia) – an AI-powered operating system for restaurants.
- Reno Home (UAE) – a renovation marketplace combining financing and vetted contractors.
- Medannot (Slovakia/Belgium) – no-code automation and AI diagnostics in radiology.
- Palm.hr (Saudi Arabia) – an HR platform for SMEs centralizing payroll and compliance.
- IntellCRE (USA/Slovakia) – AI software for commercial real estate underwriting and deal workflows.
Portfolio companies are also showing progress. Powerful Medical secured a €40M non-dilutive grant and expanded into Saudi Arabia through a strategic partnership. Filmzie marked the fund’s second exit with a 4x return. Qubu partnered with YIT Slovakia to integrate generative design into construction, while Medannot’s AI-powered visualization was recently deployed in a robotic surgery in Athens.
On the fundraising front, several startups have closed rounds, including CulturePulse ($1.5M) and Vestberry ($2.2M). Others — including PatronGo, SuperScale, Medannot, Biteberry, Powerful Medical, and Qubu — are actively seeking new investment.
Zero One Hundred will also be active on the global conference circuit, with its flagship Zero One Hundred International 2025 set for October in Milan, followed by the Doers Summit in Dubai in November.
With Fund II, the firm says it remains committed to “empowering founders beyond the check” by fostering international connections and staying closely involved in portfolio growth.
